SurePrep Plant/Fungi Total RNA Purification Kit from Fisher BioReagents

• All sizes of total RNA can be purified, from large mRNA and rRNA to small
  RNA <200 nt, such as virus RNA, miRNA, and siRNA
  
• Optimized RNA purification protocol thoroughly extracts the full complement
  of RNA from plant cells and tissues containing complex polysaccharides and
  polyphenolic substances which prove problematic in other RNA isolation
  procedures
  
• Purification based on spin column chromatography using a proprietary RNA
  af nity resin
  
• No additional steps such as salt precipitations and ultracentrifugations as
  used in other traditional RNA extraction methods
  
• No hazardous organic extractions with phenol and chloroform
  
• No liquid nitrogen to homogenize the samples

KIT SPECIFICATIONS:

Column Binding Capacity ......................................50μ g

Maximum Column Loading Volume ................600μ L

Size of RNA Purified ................................................All sizes, including small RNA

Maximum Amount of Starting Material

Plant Tissues ...........................................................50mg

Plant Cells ................................................................. 1x10⁶cells

Fungal Tissue ..........................................................50mg (wet weight)

Average Yields

50mg Tomato Leaves ..........................................60μ g

50mg Tobacco Leaves ........................................60ug

50mg Plum Leaves ...............................................32μ g

50mg Grape Leaves.............................................35μ g

50mg Peach Leaves.............................................30μ g

Time to Complete Purification Process .....30 minutes for 10 purifications